What is Alcian Blue (pH 2.5) used for?

Alcian Blue (pH 2.5) is primarily utilized for histological staining in tissue samples to identify acidic mucins and glycosaminoglycans. This stain differentiates between various tissue components, making it essential in pathology and biomedical research. For instance, it's commonly applied in cancer studies to analyze tumor tissues or in research related to embryonic development to investigate mucopolysaccharides in tissues. Its unique ability to bind to specific polysaccharides enhances the visibility of cellular structures under a microscope.

How do you prepare Alcian Blue (pH 2.5) for use?

To prepare Alcian Blue (pH 2.5), simply dilute the concentrated stain as per the provided protocol. Typically, you'll mix a specified volume of Alcian Blue with a buffer solution to achieve the desired pH. The preparation should be done in a laboratory setting equipped with appropriate safety gear. Properly prepared, it can effectively stain tissue samples, allowing for detailed examination of glycoproteins and mucins in microscopic analyses, aiding in further research studies.

What types of samples can be stained with Alcian Blue?

Alcian Blue can be applied to a range of biological samples including frozen or paraffin-embedded tissues. It's especially effective for animal tissues, plant materials or human tissue samples under examination. It can highlight features in sections of gastrointestinal tissues, connective tissues or any structures containing mucins. Researchers frequently use it to assess tissue morphology in various studies, such as investigations related to inflammatory responses or developmental biology.

Is Alcian Blue staining a complex process?

While Alcian Blue staining requires careful protocol adherence, it is generally straightforward. Key steps include preparing the samples, staining with the solution, and rinsing appropriately to reveal structures of interest. Users should follow detailed staining protocols outlined in research manuals. The process is manageable for those familiar with histological techniques, and it can significantly enhance research quality by providing clear, distinguishable tissue features for analysis and documentation.

Which specific mucins can Alcian Blue (pH 2.5) identify?

Alcian Blue (pH 2.5) is particularly proficient at highlighting acidic mucins, such as those found in goblet cells and various glycosaminoglycans. It interacts specifically with sialomucins and sulfomucins, enabling researchers to distinguish between different types of mucous products in tissues. This specificity makes it invaluable in studies related to gastrointestinal conditions, respiratory diseases, and even in cancerous tissues where mucin expression patterns can provide critical diagnostic information.

Can Alcian Blue staining be used alongside other stains?

Yes, Alcian Blue staining can be effectively combined with other histological stains, such as Hematoxylin and Eosin (H&E). This combination allows for comprehensive tissue analysis by providing both background cellular detail and highlighting specific mucins or glycosaminoglycans. Researchers often use this dual staining approach to enhance the contrast in microscopic examinations, making it easier to interpret complex tissue structures, particularly in pathological studies.

What precautions should be taken while using Alcian Blue?

When using Alcian Blue, it's crucial to wear appropriate personal protective equipment, such as gloves and lab coats, due to potential skin and eye irritation. Ensure that the workspace is well-ventilated and that you follow local guidelines for chemical use and waste disposal. Proper handling ensures that researchers can safely conduct experiments while minimizing health risks associated with chemical exposure, thus maintaining a safe laboratory environment.

How does the pH level affect Alcian Blue staining?

The pH level is critical for the effectiveness of Alcian Blue staining. At pH 2.5, the stain selectively binds to acidic mucins, revealing detailed cellular structures. Lower pH enhances the staining specificity for certain mucins, ensuring clearer visualization under a microscope. Researchers must monitor and maintain the pH during preparation and application to achieve reproducible results. Adjusting the pH may lead to variations in staining intensity, which can influence the interpretation of soft tissue analysis in various studies.

Where can I buy Alcian Blue (pH 2.5) Stain Pack, 500ml?

You can buy the Alcian Blue (pH 2.5) Stain Pack, 500ml, from Ubuy. They offer a convenient shopping experience for purchasing chemical reagents and laboratory supplies, ensuring that you have access to high-quality materials for your research and experiments. Ubuy provides an organized platform that allows for easy browsing and comparison of various lab products, making it an ideal choice for researchers and institutions in Saudi Arabia.

How to Shop Alcian Blue (pH 2.5) Stain Pack, 500ml Online From Ubuy?

It’s easy to shop Alcian Blue (pH 2.5) Stain Pack, 500ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Alcian Blue (pH 2.5) Stain Pack, 500ml Available to Shop Online in Saudi Arabia?

Yes, at Ubuy Saudi Arabia this product is available for you to shop at a reasonable price. The Alcian Blue (pH 2.5) Stain Pack, 500ml is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

Tyr Scientific LLC

Achieve stunning histological visualization of key mucopolysaccharides with our Alcian Blue Stain Pack!

Tyr Scientific LLC

Item #: 248081298

Alcian Blue (pH 2.5) Stain Pack, 500ml

Item #: 248081298

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What Stands Out

Precise pH Formula

The Alcian Blue stain is formulated at pH 2.5, ensuring accurate acid mucopolysaccharide identification in tissues, which enhances reliability in histological analysis.

Generous Volume

With 500ml of stain, this pack offers ample supply for extensive histological studies, reducing the need for frequent repurchases and ensuring consistent results across multiple experiments.

High Sensitivity

Alcian Blue's high sensitivity effectively highlights cartilage and mucus substances in biological samples, addressing the challenge of insufficient visibility often encountered with standard stains.

Product Details

Brand Name	Tyr Scientific LLC
Model Number	PH 2.5
Number of Items	1
Manufacturer	Tyr Scientific LLC
Unit Count	16.91 Fluid Ounces
Manufacturer Part Number	TS16-500PK
Container Type	Bottle
Material Type	Wood Based
Brand	Tyr Scientific LLC
Material	Wood Based
Package Information	Bottle
Item Weight	1.1 lbs (500 grams)

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Question: How to Shop Alcian Blue (pH 2.5) Stain Pack, 500ml Online From Ubuy?

Answer: It’s easy to shop Alcian Blue (pH 2.5) Stain Pack, 500ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Alcian Blue (pH 2.5) Stain Pack, 500ml Available to Shop Online in Saudi Arabia?

Answer: Yes, at Ubuy Saudi Arabia this product is available for you to shop at a reasonable price. The Alcian Blue (pH 2.5) Stain Pack, 500ml is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Features & Benefits

Designed for histological visualization of sulfated and carboxylated mucopolysaccharides.
Includes Alcian Blue Solution (pH 2.5) for vibrant blue staining.
Accompanied by Nuclear Fast Red Solution and Acetic Acid Solution for enhanced results.
Pack includes four 500ml solutions for comprehensive staining needs.
Perfect for laboratory applications and research.
Achieve precise and accurate results with our high-quality stain pack.

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Alcian Blue (pH 2.5) Stain Pack, 500ml

SAR 869

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Tyr Scientific LLC Dyes, Fluorophores & Stains PH 2.5 Editorial Review

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SAR 869

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