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Item #:
145283198
NP40 Cell Lysis Buffer, 7.4 pH, 250mL
Item #:
145283198
SAR 250
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What Stands Out
Efficient Cell Lysis
NP40 Cell Lysis Buffer effectively disrupts cell membranes, allowing for optimal extraction of cellular proteins, ensuring high yield and purity for subsequent analysis.
Neutral pH Value
With a stable pH of 7.4, this buffer maintains physiological conditions, preventing protein denaturation and ensuring reliable results in downstream applications.
Generous Volume
The 250mL packaging offers ample volume for extensive research applications, providing great value and convenience for labs requiring high-throughput cell lysis.
Product Details
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 4 ounces (113.4 grams) |
| Volume | 250 ml |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Included Components | Bottle |
| Part Number | 23.105.110 |
| Brand | eCon Lab Supply |
| Item Volume | 250 ml |
| Package Information | Bottle |
Who Should Buy?
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Molecular Biologists
Ideal for researchers needing to extract proteins and nucleic acids from various cell types efficiently.
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Cell Culture Labs
Perfect for laboratories conducting cell cultures, providing consistent results in lysis procedures.
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Biochemistry Studies
Beneficial for experiments requiring reliable disruption of cellular membranes to study metabolic pathways.
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Sensitive Samples
Not suitable for samples containing delicate proteins that may be denatured by harsh lysis conditions.
Product Description
NP40 Cell Lysis Buffer, 7.4 pH, 250mL
Have any Query? Chat with us
Customer Questions & Answers
-
Question:
What is NP40 Cell Lysis Buffer used for?
Answer: NP40 Cell Lysis Buffer is primarily used in cell biology for lysing cells to extract proteins, DNA, or RNA. This buffer facilitates the solubilization of membrane proteins and disrupts cellular structures effectively. Researchers commonly use it in applications such as Western blotting, immunoprecipitation, and nucleic acid isolation. It's particularly valuable in experiments requiring the extraction of sensitive components, ensuring the integrity of your samples during the lysis process. -
Question:
What is the pH level of NP40 Cell Lysis Buffer?
Answer: The NP40 Cell Lysis Buffer is formulated to have a pH of 7.4, which is optimal for maintaining biological activity. This neutral pH is crucial for preserving the functionality of proteins and nucleic acids during cell lysis. Using buffers at an incorrect pH can lead to protein denaturation and, consequently, experimental failure. This buffer is ideal for a variety of cellular applications, particularly when working with mammalian cell lines. -
Question:
Is NP40 Cell Lysis Buffer suitable for all cell types?
Answer: Yes, NP40 Cell Lysis Buffer is versatile and can be used with various cell types, including adherent and suspension cells. It effectively lyses different cellular structures and is particularly advantageous when working with mammalian cells. However, for very sensitive cells or specific applications, it is recommended to validate the buffer's compatibility for those particular cell types through preliminary experiments. -
Question:
How should NP40 Cell Lysis Buffer be stored?
Answer: NP40 Cell Lysis Buffer should be stored at room temperature, away from direct sunlight, to maintain its effectiveness. It is crucial to seal the container tightly to prevent contamination and evaporation. Proper storage helps in preserving the integrity of chemical components, ensuring optimal performance in cell lysis applications when you need to use it later. -
Question:
Can NP40 Cell Lysis Buffer extract RNA?
Answer: Absolutely, NP40 Cell Lysis Buffer can effectively extract RNA from cells. The buffer's composition allows for the disruption of cellular components while preserving RNA integrity. This characteristic is essential for subsequent applications such as RT-PCR or RNA sequencing. Ensure to follow appropriate protocols to isolate RNA without contamination from proteins or other cellular debris. -
Question:
What are the components of NP40 Cell Lysis Buffer?
Answer: NP40 Cell Lysis Buffer generally contains non-ionic detergents, salts, and buffering agents. The primary component, NP40 (Nonidet P-40), allows for the solubilization of proteins while maintaining their functional structure. Understanding the components helps in modifying the buffer for specialized applications or in troubleshooting when results are not as expected during an experiment. -
Question:
Can I use NP40 Cell Lysis Buffer for protein extraction?
Answer: Yes, NP40 Cell Lysis Buffer is an excellent choice for protein extraction from cells. Its formulation helps solubilize membrane proteins and release cytoplasmic proteins from the cells. This makes it a preferred buffer in proteomics studies, enabling researchers to analyze protein expression and modifications. For the best results, always optimize the lysis conditions based on your specific cell type and experiment. -
Question:
What precautions should be taken while using NP40 Cell Lysis Buffer?
Answer: When using NP40 Cell Lysis Buffer, it is critical to wear appropriate personal protective equipment (PPE), such as gloves and goggles, as it may irritate skin and eyes. Additionally, avoid using it with extreme temperatures or in concentrated forms; always dilute as per your experimental needs. These precautions ensure safety and maintain the integrity of your samples during the lysis process. -
Question:
How is NP40 Cell Lysis Buffer different from other lysis buffers?
Answer: NP40 Cell Lysis Buffer differentiates itself from other lysis buffers by its non-ionic detergent, which minimizes protein denaturation while allowing efficient lysis of cells. Other buffers may contain ionic detergents that can precipitate proteins and disrupt protein-protein interactions. NP40 is particularly beneficial for applications requiring gentle lysis, making it suitable for analyses such as immunoprecipitation and protein assays. -
Question:
Where can I buy NP40 Cell Lysis Buffer, 7.4 pH, 250mL?
Answer: You can conveniently purchase NP40 Cell Lysis Buffer, 7.4 pH, 250mL from Ubuy. They offer a wide selection of laboratory supplies, including various biochemical reagents, ensuring you have the necessary tools for your research needs.
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Features & Benefits
- Produced in a certified facility ensuring high standards.
- Meets stringent Good Manufacturing Practices.
- Ideal for preparing cell extracts for various assays.
- Compatible with Antibody Bead Immunoassay, ELISA, and Western blotting.
- Can be used as a wash buffer for immunoprecipitation reactions.
- Completely soluble in water for easy use.
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