0 ratings
![Tris Buffer, pH 8.5 [1M] - 125 mL Volume](https://m.media-amazon.com/images/I/31Hra4HFjcL.jpg)
![Tris Buffer, pH 8.5 [1M] - 125 mL Volume](https://www.ubuy.com.sa/productimg/?image=aHR0cHM6Ly9tLm1lZGlhLWFtYXpvbi5jb20vaW1hZ2VzL0kvMzFIcmE0SEZqY0wuanBn.jpg)
Item #:
145280186
Tris Buffer, pH 8.5 [1M] - 125 mL Volume
82% of respondents would recommend this to a friend
Item #:
145280186
SAR 195
Price Details
Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )
*All items will import from US
0 ratings
Write a review
In stock
Imported from USA store
QTY:
Order now and get it around Sunday, September 13
Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
Our Top Logistics Partners
1M Tris Buffer pH 8.0 is a ready-to-use solution essential for your molecular biology and proteomics applications.
Buy Now Pay Later
Fast
Shipping
Free
Return*
Secure Packaging
100% Original Products
PCI DSS Compliance
ISO 27001 Certified
Note: Step Down Voltage Transformer required for using electronics products of US store (110-120). Recommended power converters Buy Now.
What Stands Out
Standardization
This Tris Buffer offers a consistent pH of 8.5, ensuring reliable results in biochemical experiments and reactions that require precise buffering capacity.
High Concentration
With a 1M concentration, this buffer provides ample capacity to maintain stable pH levels in various applications, making it suitable for high-demand laboratory needs.
Convenient Volume
Packaged in a 125 mL bottle, it strikes a balance between sufficient quantity for experiments and usability, preventing waste while catering to both small and medium-scale applications.
Product Details
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 0.25 Pounds (110 grams) |
| Volume | 125 Milliliters |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.35 |
| Included Components | Bottle |
| Brand | eCon Lab Supply |
| Item Volume | 125 Milliliters |
| Package Information | Bottle |
Who Should Buy?
-
Biochemistry Labs
Ideal for researchers conducting experiments that require stable pH conditions in biochemical reactions.
-
PCR Applications
Useful for molecular biologists performing PCR, as it helps maintain optimal pH during DNA amplification.
-
Cell Culture
Perfect for cell biologists needing a buffer for maintaining pH in cell culture mediums.
-
Acidic Solutions
Not suitable for applications requiring acidic conditions, as Tris buffer is not effective below pH 7.
Product Description
Tris Buffer, pH 8.5 [1M] - 125 mL Volume
Have any Query? Chat with us
Customer Questions & Answers
-
Question:
What is Tris buffer and what is its purpose?
Answer: Tris buffer, also known as Tris(hydroxymethyl)aminomethane, is a widely used buffering agent in biological and chemical applications. Its primary purpose is to maintain a consistent pH during experiments, particularly in molecular biology, biochemistry, and electrophoresis. Tris buffer at pH 8.5 is particularly ideal for experiments requiring basic conditions, ensuring that enzymes and proteins remain stable. For instance, researchers often employ this buffer when conducting PCR reactions or protein assays, where precise pH control can significantly affect the outcomes. -
Question:
How do I prepare Tris buffer at pH 8.5?
Answer: Preparing Tris buffer at pH 8.5 is straightforward. Start with Tris base and deionized water, then adjust the pH by adding hydrochloric acid (HCl) until you reach the desired level. For a ready-to-use solution, you can purchase pre-made Tris buffer like the 1M version in a 125 mL volume. This convenience eliminates the need for complex calculations and ensures consistency across experiments, making it ideal for time-sensitive work like gel electrophoresis, where reliable pH is essential for separation processes. -
Question:
Is Tris buffer safe to use in the lab?
Answer: Yes, Tris buffer is generally regarded as safe for laboratory use when handled with appropriate lab safety protocols. It has low toxicity, making it favorable for many biological applications. However, as it is a chemical reagent, it's important to avoid direct contact with skin and eyes and to work in a well-ventilated area. In practical scenarios, researchers often utilize Tris buffer for sensitive experiments involving DNA, RNA, or proteins, ensuring a safe environment while yielding reliable results in their studies. -
Question:
What applications is Tris buffer used for?
Answer: Tris buffer is highly versatile and is used in a variety of applications spanning molecular biology, protein synthesis, and electrophoresis. It's commonly applied in DNA and RNA extraction processes, as well as in the preparation of agarose gels for electrophoresis. This buffer is also used in cell culture and enzymatic reactions where maintaining a stable pH is crucial. For example, in Western blotting, Tris buffer helps to ensure the integrity of protein separation, enhancing assay reproducibility and accuracy. -
Question:
Can Tris buffer be used with all types of samples?
Answer: While Tris buffer is suitable for a wide range of biological samples, it's important to note that it may not be compatible with all. Certain proteins or enzymatic reactions can be sensitive to Tris, which may interfere with assay results. For example, if conducting a project that involves proteins sensitive to basic pH, it’s advisable to evaluate alternative buffers. Researchers often select Tris buffer for nucleic acid work or protein studies, given its broad applicability and reliable performance in these scenarios. -
Question:
How long can I store Tris buffer?
Answer: Tris buffer can typically be stored for a prolonged period, often up to several months if kept under proper conditions. It should be stored in a cool, dark place and ideally in a tightly sealed container to prevent contamination and evaporation. However, it is crucial to regularly check for any color changes or precipitation, which can indicate degradation. In laboratory contexts, it is common for scientists to prepare fresh buffer solutions as needed for critical experiments to ensure optimal performance. -
Question:
What is the significance of the pH level in Tris buffer?
Answer: The pH level in Tris buffer is crucial as it impacts the stability and activity of biological molecules, such as proteins and nucleic acids. A pH of 8.5 is optimal for many biochemical applications because it closely resembles physiological conditions in many organisms. Changes in pH can lead to denaturation or loss of activity in sensitive applications. Therefore, when using Tris buffer in experiments such as enzyme assays or DNA amplification, maintaining the precise pH is essential for achieving reliable results. -
Question:
Can Tris buffer be used for preparing gels?
Answer: Absolutely, Tris buffer is commonly used in preparing agarose and polyacrylamide gels for electrophoresis. Its ability to maintain a stable pH during the electrophoretic process is essential for consistent banding patterns and resolution. When preparing these gels, scientists typically mix Tris buffer with agarose or acrylamide, depending on the type of samples they're working with. This practice is fundamental in molecular biology labs for analyzing DNA, RNA, or proteins, facilitating clear visualization and accurate separation during analysis. -
Question:
Where can I buy Tris Buffer, pH 8.5 [1M] - 125 mL Volume?
Answer: You can purchase Tris Buffer, pH 8.5 [1M] - 125 mL Volume from Ubuy in Saudi Arabia. Ubuy provides a convenient online shopping experience, ensuring that you have access to quality laboratory products like Tris buffer. They often stock various sizes and concentrations, allowing you to choose the most suitable option for your research needs. With Ubuy's extensive range and reliable service, it's an excellent choice for obtaining your lab supplies efficiently.
eCon Lab Supply Buffers Editorial Review
No editorial reviews found
Customer Reviews & Ratings
1 customers ratings
-
5 Star
100%
-
4 Star
0%
-
3 Star
0%
-
2 Star
0%
-
1 Star
0%
Review this product
Share your thoughts with other customers
Platform Trust & Buyer Confidence
MA
Mekki
Verified buyer
“Arrive before time, excellent products”
9 July 2026 · via Trustpilot
FS
Farrah
Verified buyer
“A very big Thank you to HUDA and the Ubuy Team in helping to send my order to me yesterday!! Order # 62606425316 Customer service and operations is top for this online shopping company and I am very satisfied with the way they handle issues. THANK YOU once again!!”
25 June 2026 · via Trustpilot
AF
Anila
Verified buyer
“Good experience of shopping through Ubuy.”
16 June 2026 · via Trustpilot
MG
Mohamed
Verified buyer
“We feel very good about the delivery time, and the materials we received matched our order.”
14-Jul-26 · via Trustpilot
E
Essam
Verified buyer
“Perfect service and fast delivery.”
16-Jul-26 · via Trustpilot
Secure Checkout
Global Delivery
Easy Returns
Genuine Products
Product Price History
Important information
- Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
- Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.
SAR 195
Order now and get it around Sunday, September 13
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
QTY:
Secured transaction
PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.
Our Top Logistics Partners
Fastest cross-border delivery
Features & Benefits
- Comprehensive range of biological buffers for research and diagnostics.
- Manufactured in an ISO certified facility in the USA.
- Strict adherence to Good Manufacturing Practices and quality control.
- 1M Tris Buffer pH 8.0 is ready-to-use and versatile.
- Prepared with high-quality molecular biology grade ingredients.
- Suitable for both DNA and RNA applications.
Ubuy Assurance
Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns *, and secure packaging on every order.