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Item #:
182389312
Phosphate Buffer, pH 6.0 [0.5 M] - 250 ml
Item #:
182389312
SAR 235
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What Stands Out
Optimal pH Stability
This phosphate buffer maintains a precise pH of 6.0, ensuring reliable conditions for biological and chemical applications, enhancing experiment accuracy and consistency.
High Concentration
At 0.5 M concentration, this buffer delivers effective buffering capacity, ideal for various laboratory procedures, ensuring reliable results in complex reactions and solutions.
Convenient Volume
Available in a 250 ml package, this phosphate buffer is perfect for routine lab work, reducing the need for frequent preparation, saving time and increasing laboratory efficiency.
Product Details
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 0.5 Pounds (230 grams) |
| Volume | 250 Milliliters |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.74 |
| Included Components | Bottle |
| Brand | eCon Lab Supply |
| Item Volume | 250 Milliliters |
| Package Information | Bottle |
Who Should Buy?
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Laboratory Experiments
Ideal for biochemical and biological experiments that require a stable pH environment for reactions and assays.
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Cell Culture
Supports maintaining the desired pH level in cell culture applications, promoting optimal growth conditions for sensitive cells.
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Analytical Applications
Useful in analytical chemistry for calibrating instruments or performing pH-sensitive analyses that require precision.
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High-Temperature Use
Not suitable for high-temperature applications where buffer stability and performance may be compromised.
Product Description
Phosphate Buffer, pH 6.0 [0.5 M] - 250 ml
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Customer Questions & Answers
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Question:
What is a Phosphate Buffer, pH 6.0 [0.5 M] used for?
Answer: A Phosphate Buffer, pH 6.0 [0.5 M] is primarily used in biological and chemical applications to maintain stable pH levels during experiments. It is particularly beneficial in enzymatic reactions, where a consistent pH is crucial for optimal activity. By providing an environment that resists changes in pH, it ensures accurate results in research, cell culture, and molecular biology applications. -
Question:
How do you prepare a Phosphate Buffer, pH 6.0 [0.5 M]?
Answer: Preparation of a Phosphate Buffer at pH 6.0 involves mixing specific concentrations of potassium dihydrogen phosphate (KH2PO4) and disodium hydrogen phosphate (Na2HPO4). The buffer solution maintains the desired pH through the equilibrium between these phosphate components. This preparation is essential in various laboratory settings where pH control is vital for reproducibility in experimental results. -
Question:
Can I use Phosphate Buffer for cell culture applications?
Answer: Yes, Phosphate Buffer, pH 6.0 [0.5 M] is widely used in cell culture applications. The buffer helps maintain physiological pH levels, which is critical for cell growth and viability. It ensures that the medium remains stable, minimizing fluctuations that could stress the cells and affect their growth. This makes it an essential component in preparing culture media. -
Question:
Is Phosphate Buffer suitable for biochemical assays?
Answer: Absolutely, Phosphate Buffer, pH 6.0 [0.5 M] is extensively used in biochemical assays. Its ability to maintain a stable pH environment is crucial for precise measurements and reactions, especially in enzyme kinetics and other assays that depend on specific pH levels for accurate results. Researchers rely on this buffer to reduce variability in their experimental outcomes. -
Question:
What are the storage conditions for Phosphate Buffer?
Answer: It is important to store Phosphate Buffer, pH 6.0 [0.5 M] in a cool, dark place, ideally between 2-8°C, to maintain its stability and effectiveness. Proper storage prevents degradation and ensures that the buffer retains its pH properties. For laboratory usage, transferring it to appropriate, sterilized containers can extend its usability. -
Question:
Can I use Phosphate Buffer for biological sample preservation?
Answer: Phosphate Buffer can be an effective choice for preserving biological samples. By maintaining a stable pH, it helps in minimizing degradation of biomolecules during storage. This is particularly useful for sensitive biological samples in microscopy or analytical procedures, where pH fluctuations could alter cellular components or lead to loss of activity. -
Question:
What is the importance of pH 6.0 specifically in buffers?
Answer: The pH of 6.0 is significant because it closely resembles physiological conditions of many biological systems. This pH level is optimal for various biochemical reactions and closely mirrors conditions found in human tissues. By using a Phosphate Buffer at this pH, researchers and laboratory professionals can ensure that their experiments more accurately reflect real biological environments. -
Question:
What types of experiments typically require a phosphate buffer?
Answer: Phosphate Buffers are often used in a variety of experiments, including enzymatic assays, microbial growth studies, and molecular biology procedures such as DNA extraction. Any experiment that requires strict control of pH over a range of physiological processes will benefit from using this buffer, leading to more reliable and reproducible results. -
Question:
Can I use this buffer for protein purification processes?
Answer: Yes, Phosphate Buffer, pH 6.0 [0.5 M] is suitable for protein purification as it provides an optimal pH environment that preserves protein structure and function. This buffer is integral in chromatography techniques and can help maintain solubility, reducing protein aggregation. Thus, it plays a vital role in obtaining high-purity protein samples for further analysis. -
Question:
Where can I buy Phosphate Buffer, pH 6.0 [0.5 M] - 250 ml in Saudi Arabia?
Answer: You can buy Phosphate Buffer, pH 6.0 [0.5 M] - 250 ml from Ubuy. They offer a wide range of laboratory supplies and ensure a convenient online shopping experience, making it easy to find the buffers needed for your experiments.
eCon Lab Supply Buffers Editorial Review
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Pros
- High stability at pH 6.0
- Ideal for various experiments
- Easy to prepare and use
- Reliable for biological applications
- Non-toxic and safe handling
Cons
- The bottle size may be too small for some.
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“We feel very good about the delivery time, and the materials we received matched our order.”
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Features & Benefits
- Comprehensive range of biological buffers for various uses.
- Ideal for research, diagnostics, and drug development.
- Manufactured in ISO certified facility ensuring high quality.
- Produced under stringent Good Manufacturing Practices.
- Phosphate buffer is essential for immunochemical staining.
- Buffers maintain pH stability, ideal for physiological conditions.
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