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Item #:
175546200
Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml
Item #:
175546200
SAR 235
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Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers offer unparalleled quality for your molecular biology research.
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What Stands Out
Optimal pH Stability
This phosphate buffer maintains a stable pH of 7.2, ensuring reliable results in various biological and chemical experiments, reducing the risk of fluctuations that could compromise study integrity.
Concentrated Formula
With a concentration of 1.0 M, this buffer allows for easy preparation of dilutions, facilitating precise and controlled experimental conditions crucial for research and laboratory work.
Versatile Applications
Ideal for molecular biology and biochemical assays, this buffer is compatible with a wide range of applications, ensuring flexibility and reliability for lab professionals across various research fields.
Product Details
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Included Components | Bottle |
| Part Number | 23.105.88 |
| Item Weight | 8 ounces (226.8 grams) |
| Brand | eCon Lab Supply |
Who Should Buy?
-
Biologists
Ideal for experiments requiring stable pH conditions, ensuring optimal enzyme activity and accurate molecular biology workflows.
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Chemists
Useful for maintaining pH in various chemical reactions, enhancing reliability in laboratory experiments and formulations.
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Healthcare labs
Essential for diagnostic procedures or assays that require precise pH levels, improving test accuracy and patient outcomes.
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Educational use
May not suit beginners as handling buffers requires some chemistry knowledge and experience for safety and effectiveness.
Product Description
Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml
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Customer Questions & Answers
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Question:
What is a Phosphate Buffer, pH 7.2 used for?
Answer: A Phosphate Buffer, pH 7.2 is commonly used in biological and chemical laboratories to maintain a stable pH environment essential for various experiments. This buffer solution is particularly beneficial in enzymatic reactions, cell culture, and molecular biology applications where pH fluctuation could impact the outcome. For instance, many enzymes exhibit optimal activity at specific pH levels, and using this buffer can help maintain those conditions. -
Question:
How do you prepare a Phosphate Buffer, pH 7.2?
Answer: To prepare a Phosphate Buffer, pH 7.2, a precise mixture of disodium hydrogen phosphate (Na2HPO4) and monosodium phosphate (NaH2PO4) is required. This involves dissolving a calculated amount of each salt in distilled water, and adjusting the pH to 7.2 using a pH meter. This method is crucial for applications requiring accurate pH values, such as biochemical assays or cell culture media preparations. -
Question:
What are the advantages of using a Phosphate Buffer?
Answer: Phosphate Buffers are widely favored due to their ability to maintain stable pH levels with minimal changes upon dilution or during ionic strength variations. They are effective in a physiological range, making them ideal for biochemical reactions that mimic in vivo conditions, such as protein purification and cellular experiments. Additionally, they exhibit low absorbance at UV wavelengths, making them suitable for spectrophotometric analysis. -
Question:
Can I use Phosphate Buffer for cell culture?
Answer: Yes, Phosphate Buffer, pH 7.2 is frequently used in cell culture applications. It helps maintain the physiological pH that is crucial for cell viability and function. In cell culture media, this buffer plays a vital role in providing the stability needed for optimal cell growth and productivity, allowing researchers to conduct experiments without adverse effects from pH fluctuations. -
Question:
Is Phosphate Buffer suitable for protein assays?
Answer: Absolutely, Phosphate Buffer, pH 7.2 is highly suitable for protein assays. Many analytical methods, such as enzyme-linked immunosorbent assays (ELISA) or spectrophotometric analyses, rely on a stable pH environment to ensure accurate results. The buffer's ability to maintain consistent pH levels helps ensure that the proteins retain their biological activity and structural integrity during analysis. -
Question:
What should I consider when storing Phosphate Buffer?
Answer: When storing Phosphate Buffer, pH 7.2, it's important to keep it in a tightly sealed container away from direct light and temperature extremes. Exposure to air may lead to contamination or evaporation, altering its pH. Proper storage ensures that the buffer remains effective for laboratory use, especially for sensitive experiments requiring pH control. -
Question:
Can Phosphate Buffer be used in chemical synthesis?
Answer: Yes, Phosphate Buffer, pH 7.2 can be utilized in various chemical synthesis processes. It can help in maintaining the desired pH during reactions involving acid-base chemistry, where a stable pH is crucial for the desired outcomes. This buffer is often employed in reactions such as esterification or polymerization, where pH stability influences the reaction rate and mechanism. -
Question:
Is Phosphate Buffer compatible with all biochemical reactions?
Answer: While Phosphate Buffer is versatile, it's not suitable for all biochemical reactions. Some reactions may be sensitive to phosphate ions or require a different pH range. For instance, reactions involving magnesium-dependent enzymes may necessitate using alternative buffering systems. Therefore, it's essential to evaluate the specific requirements of your experiment to ensure compatibility. -
Question:
What is the concentration of Phosphate Buffer, pH 7.2?
Answer: The Phosphate Buffer, pH 7.2 is available in a concentration of 1.0 M, which indicates that it contains 1 mole of phosphate ions per liter. This concentration is often preferred for laboratory applications because it provides effective buffering capacity and maintains stability against pH changes during biochemical processes. Higher concentrations might be too strong for some applications, while lower concentrations could compromise the buffering ability. -
Question:
Where can I buy Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml?
Answer: You can easily purchase Phosphate Buffer, pH 7.2 [1.0 M] - 250 ml from Ubuy in Saudi Arabia. Ubuy offers a wide selection of laboratory reagents, including this buffer solution, ensuring a convenient shopping experience for your laboratory needs.
eCon Lab Supply Buffers Editorial Review
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Pros
- Highly effective pH stabilization
- Pre-made for convenience
- Reliable for laboratory use
- consistent quality and performance
- Suitable for various applications
Cons
- Bottle design could be improved.
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Features & Benefits
- Comprehensive range of biological buffers for research and diagnostics.
- Ideal for protein research, molecular biology, and characterization.
- Manufactured in USA in ISO 9001 and 13485 certified facility.
- Produced under stringent Good Manufacturing Practices.
- Quality control tests conducted in certified 14644 clean room conditions.
- Perfect for immunochemical staining and preserving living tissues.
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