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112933250
Bio Basic BS614 EZ-10 Spin Column Plasmid DNA Mini-Preps Kit, 250 Preps
83% of respondents would recommend this to a friend
Item #:
112933250
SAR 749
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The ez-10 spin column kits provide a simple and efficient method for purification of plasmid DNA and more.
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What Stands Out
High Throughput
The BS614 EZ-10 kit allows for 250 plasmid preparations, ideal for labs needing efficient and scalable solutions without compromising on performance.
User-Friendly Design
Designed for simplicity, the EZ-10 kit offers streamlined protocols, making it easy for users of all experience levels to achieve reliable results rapidly.
Quality Purification
Delivers high-purity plasmid DNA, ensuring consistent quality for downstream applications, surpassing many competitors in reliability and yield.
Product Details
Who Should Buy?
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Molecular Biologists
Ideal for molecular biologists needing quick, reliable plasmid isolation from bacterial cultures for cloning or sequencing.
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Academic Researchers
Perfect for researchers in academic settings conducting experiments requiring multiple plasmid extractions efficiently.
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Small Labs
Suited for small laboratory setups that require a cost-effective solution for a high number of plasmid preparations.
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Large Scale Production
Not suitable for users needing high-volume plasmid extraction due to its mini-prep scale limitations.
Product Description
Bio Basic BS614 EZ-10 Spin Column Plasmid DNA Mini-Preps Kit, 250 Preps
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Customer Questions & Answers
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Question:
What is the BS614 EZ-10 Spin Column Plasmid DNA Mini-Preps Kit used for?
Answer: The BS614 EZ-10 Spin Column Plasmid DNA Mini-Preps Kit is designed for the rapid and efficient isolation of plasmid DNA from bacterial cultures. This kit allows researchers to easily prepare high-quality plasmid DNA for various applications such as cloning, sequencing, or transfection. Its spin column format ensures quick separation without the need for cumbersome protocols, streamlining your workflow in molecular biology tasks. -
Question:
How does the EZ-10 Spin Column work?
Answer: The EZ-10 Spin Column utilizes a silica-based membrane that selectively binds DNA under high-salt conditions, allowing impurities, such as proteins and other contaminants, to be washed away. After binding the plasmid DNA to the column, a simple washing step ensures maximum purity before elution. This method is effective for small to medium plasmid preparations, making it suitable for routine laboratory use where time and efficiency are critical. -
Question:
How many preps are included in the kit?
Answer: The BS614 EZ-10 Kit is designed for 250 plasmid DNA preps. This bulk quantity is ideal for laboratories or research projects that require multiple isolations over time. The high throughput reduces the need for multiple orders, making it an economical choice for consistent plasmid isolation while maintaining laboratory efficiency. -
Question:
Can this kit be used with low-copy plasmids?
Answer: Yes, the BS614 EZ-10 Spin Column Plasmid DNA Mini-Preps Kit is effective with both low-copy and high-copy plasmids. For low-copy plasmids, it is recommended to increase the culture volume to enhance the yield. This versatility makes the kit suitable for different experimental setups and ensures reliable results across various plasmid types used in research. -
Question:
What is the expected yield from this kit?
Answer: The yield from the BS614 EZ-10 Kit typically ranges from 20 µg to 30 µg of plasmid DNA per prep, depending on the bacterial strain and culture conditions used. For optimal results, it's essential to use well-established protocols and adequate culture growth, ensuring that the isolated plasmid is of high purity and suitable for downstream applications. -
Question:
Is the plasmid DNA isolated suitable for sequencing?
Answer: Yes, the plasmid DNA isolated using the BS614 EZ-10 Kit is of high quality and suitable for sequencing applications. The kit’s purification process effectively removes contaminants that could interfere with sequencing results, making it a reliable source for obtaining plasmid DNA for applications in genomic studies, functional genomics, or synthetic biology. -
Question:
Can this kit be used for both high-throughput and individual preps?
Answer: Absolutely! The BS614 EZ-10 Spin Column Plasmid DNA Mini-Preps Kit can accommodate both high-throughput applications and individual preps. For laboratories conducting numerous experiments simultaneously, this kit's efficiency allows for processing many samples at once. Conversely, for single sample requirement, the streamlined protocol still leads to consistent, reliable output. -
Question:
What buffers are included in the kit?
Answer: The BS614 EZ-10 Kit includes all the necessary buffers required for the entire plasmid isolation process, including buffer solutions for cell lysis, neutralization, and washing. Each buffer is formulated to optimize plasmid binding, ensuring efficient purification while minimizing contaminant co-elution. This comprehensive setup saves time and ensures that researchers have everything they need at their fingertips. -
Question:
How should I store the kit components?
Answer: It is recommended to store the components of the BS614 EZ-10 Kit at -20°C to maintain their stability and effectiveness. Keeping the buffers at the right temperature prevents degradation and ensures that your plasmid isolations remain reliable. Proper storage extends the shelf-life of your kit and maintains the integrity of your results across experiments. -
Question:
Where can I buy the BS614 EZ-10 Spin Column Plasmid DNA Mini-Preps Kit in Saudi Arabia?
Answer: You can purchase the BS614 EZ-10 Spin Column Plasmid DNA Mini-Preps Kit at Ubuy in Saudi Arabia. Ubuy offers a wide selection of laboratory supplies, making it a convenient online destination for researchers looking to obtain this essential kit for their plasmid DNA preparations.
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Features & Benefits
- Simple and efficient DNA purification method.
- Suitable for plasmid DNA and agarose gel extraction.
- Effective for purification from PCR and enzyme reactions.
- User-friendly design for convenience.
- High-quality product made in the United States.
- Perfect for researchers and laboratories.
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