Item #:
125665253
10x TBS Buffer 1 L, wKCl
96% of respondents would recommend this to a friend
Item #:
125665253
SAR 213
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TRIS-buffered saline (10x TBS) acts as a pH stabilizer, allowing for washing without disruption of antibody-antigen binding interactions.
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What Stands Out
Concentration
Highly concentrated 10x TBS buffer allows for easy dilution, ensuring versatility in various laboratory applications to meet specific experimental needs.
Potassium Chloride
Contains KCl to maintain ionic strength, stabilizing protein interactions and enhancing performance in biochemical assays compared to standard buffers.
Volume Convenience
Offered in a 1-liter volume, ideal for high-throughput applications, reducing the frequency of ordering and preparation for busy laboratories.
Product Details
Who Should Buy?
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Molecular Biologists
Ideal for PCR, western blotting, and other applications requiring stable pH and ionic strength.
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Research Laboratories
Essential for experiments needing consistent buffer conditions to ensure reproducibility of results.
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Academic Institutions
Useful for teaching purposes in biochemistry and molecular biology courses, enhancing practical lab experiences.
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Beginner Users
May be challenging for novices who lack experience with buffer preparations and biochemical applications.
Product Description
10x TBS Buffer 1 L, wKCl
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Customer Questions & Answers
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Question:
What is 10x TBS Buffer (1 L, w/KCl) used for?
Answer: 10x TBS Buffer is widely utilized in molecular biology laboratories primarily for the preparation of dilutions and buffer systems in various experiments. Its formulation includes Tris and NaCl, which help maintain a stable pH and ionic strength, providing an optimal environment for biochemical reactions. It is commonly employed in western blots, DNA/RNA extraction, and other applications where maintaining the integrity of nucleic acids or proteins is critical. -
Question:
How do I prepare 1x TBS Buffer from 10x TBS Buffer?
Answer: To prepare a 1x TBS Buffer from a 10x solution, carefully dilute the buffer by mixing one part of 10x TBS with nine parts of distilled water. For instance, if you need 100 mL of 1x TBS, combine 10 mL of 10x TBS with 90 mL of distilled water. This ensures you achieve the desired concentration for your experiments, maintaining optimal conditions for reactions. -
Question:
What is the role of KCl in 10x TBS Buffer?
Answer: KCl in 10x TBS Buffer contributes to the buffer's ionic strength, which is crucial in stabilizing macromolecules like proteins and nucleic acids during various biological assays. This enhances the buffer's ability to maintain a consistent pH, ensuring that enzymatic reactions occur effectively. The presence of potassium ions also helps with the stabilization of nucleic acids, making it a valuable component in molecular biology applications. -
Question:
Can 10x TBS Buffer be used for cell culture applications?
Answer: While 10x TBS Buffer is not primarily designed for cell culture, it can be utilized for washing and resuspending cells during experiments involving protein analysis. Proper dilution is essential, as high concentrations may negatively impact cell viability. In a typical application, researchers may use diluted TBS buffer to wash cells before lysis in protein extraction protocols. -
Question:
Is 10x TBS Buffer compatible with enzyme assays?
Answer: Yes, 10x TBS Buffer is compatible with a variety of enzyme assays. Its buffered nature provides a stable pH necessary for enzyme activity in biochemical reactions. However, to avoid interference, it is recommended to assess compatibility with specific enzymes, as certain reactions may be sensitive to buffer components. Researchers often use this buffer in enzyme-linked immunosorbent assays (ELISAs) and other similar applications. -
Question:
What precautions should I take when handling 10x TBS Buffer?
Answer: When handling 10x TBS Buffer, it is essential to wear appropriate personal protective equipment (PPE), including gloves and goggles, to avoid skin and eye contact. Additionally, ensure that the buffer is stored properly, away from direct sunlight and at room temperature, to maintain its integrity. Following standard laboratory safety protocols will help prevent contamination and ensure accurate experimental results. -
Question:
How long can I store 10x TBS Buffer?
Answer: 10x TBS Buffer can typically be stored for several months when kept in appropriate conditions. Store it at room temperature and protect it from light to prevent degradation. Always ensure that the container is tightly sealed to avoid contamination. If there are visible changes in color or turbidity, it is advisable to prepare a fresh solution for optimal experimental performance. -
Question:
Does 10x TBS Buffer affect protein stability?
Answer: 10x TBS Buffer is formulated to maintain protein stability by providing a controlled pH and ionic environment. The buffer's components help minimize degradation and denaturation of proteins during experiments. Researchers frequently use this buffer in procedures such as western blotting and protein assays where preserving protein integrity is crucial for obtaining reliable results. -
Question:
Where can I buy 10x TBS Buffer (1 L, w/KCl) in Saudi Arabia?
Answer: You can buy 10x TBS Buffer (1 L, w/KCl) from Ubuy. They offer a reliable platform for laboratory supplies, ensuring that you have access to quality products readily available in Saudi Arabia. Simply visit their website and search for the buffer to make your purchase.
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Features & Benefits
- Essential for Western blot and ELISA applications.
- Prevents disruption of antibody-antigen interactions.
- Ready to use upon dilution to desired concentration.
- Clear and colorless liquid for easy handling.
- High-quality composition ensures reliable results.
- pH balanced for optimal performance in assays.
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