Item #:
125664602
20x TBST Buffer 4 L, w/KCl
Item #:
125664602
SAR 817
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TRIS-buffered saline with Tween-20 is your essential washing buffer for preserving antibody-antigen interactions in immunoassays.
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What Stands Out
High Concentration
The 20x TBST buffer provides a highly concentrated solution, ensuring optimal performance in various biochemical applications, leading to more effective results in experiments and diagnostics.
Convenient Volume
Offered in a 4-liter volume, this buffer solution allows users to have a substantial supply for repeated experiments, minimizing the need for frequent repurchases and maximizing productivity.
Potassium Chloride
Formulated with KCl, this TBST buffer enhances stability and improves ionic strength, making it ideal for various laboratory techniques, ensuring reliable and reproducible results across applications.
Product Details
| Brand Name | Generic |
| Manufacturer | Bioland Scientific LLC |
| Model Number | TBST04-4L |
| Part Number | TBST04-4L |
| Included Components | buffer |
| Item Weight | 9 lbs (4.08 kg) |
Who Should Buy?
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Laboratory Researchers
Ideal for researchers conducting western blotting, immunohistochemistry, and related assays requiring optimized buffer conditions.
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Biotech Companies
Useful for biotech firms producing reagents, enabling consistent results in protein analysis and purification tasks.
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Educational Institutions
Perfect for universities and colleges teaching biochemistry or molecular biology with hands-on lab exercises.
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Home Users
Not suitable for non-professionals or casual DIY projects due to the required technical expertise.
Product Description
20x TBST Buffer 4 L, w/KCl
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Customer Questions & Answers
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Question:
What is 20x TBST Buffer (4 L, w/KCl) used for?
Answer: 20x TBST Buffer is primarily used in various biological and biochemical applications, particularly in Western blotting and immunodetection. The buffer maintains a consistent pH level which is crucial for the stability of proteins during experiments. The presence of KCl enhances ionic strength, which improves the binding of antibodies. Researchers often use it to dilute antibodies or wash membranes, ensuring efficient signal detection in applications like protein analysis and cell interaction studies. -
Question:
How should I store 20x TBST Buffer?
Answer: 20x TBST Buffer should be stored at a temperature range of 2°C to 8°C for optimal longevity and stability. Keeping the buffer refrigerated helps preserve its quality and effectiveness for experimental use. Additionally, it’s best to keep it away from direct light and extreme temperature fluctuations to prevent degradation. Proper storage conditions ensure that the buffer remains functional, allowing researchers to achieve reliable results during experiments involving protein assays. -
Question:
Can I use 20x TBST Buffer with other solutions?
Answer: Yes, 20x TBST Buffer can be used in conjunction with other solutions, particularly for diluting proteins or antibodies. However, it’s important to ensure compatibility with the specific reagents you intend to use. Mixing with incompatible solutions might affect the buffer’s performance and efficacy. Researchers often blend it with blocking agents or other buffers to achieve optimal conditions in assays such as ELISA or Western blotting, enhancing specificity and reducing background noise. -
Question:
Is 20x TBST Buffer suitable for mammalian cell experiments?
Answer: Yes, 20x TBST Buffer is suitable for experiments involving mammalian cells, serving as an effective washing and dilution medium. Its composition, particularly with KCl, supports cell membrane integrity and protein binding, which is crucial for accurate analysis. When researchers conduct assays using mammalian cells, utilizing TBST Buffer helps facilitate stringent washing steps, thus improving assay sensitivity and specificity for reliable data analysis in protein expression studies. -
Question:
What is the concentration factor of 20x TBST Buffer?
Answer: The concentration factor of 20x TBST Buffer indicates that it is 20 times more concentrated than the working solution, typically used in a 1x dilution for practical use. This high concentration allows laboratories to prepare a smaller volume of working solution and reduces waste. Researchers often dilute it with distilled water or other buffers to achieve the desired final concentration while ensuring that the components remain effective for binding and detection in studies. -
Question:
What are the components of 20x TBST Buffer?
Answer: 20x TBST Buffer comprises Tris base, sodium chloride, and Tween 20, with the addition of potassium chloride (KCl). Tris acts as a buffering agent to maintain pH, sodium chloride helps in maintaining the osmotic balance, and Tween 20 is a non-ionic surfactant that minimizes non-specific binding. The inclusion of KCl enhances ionic strength, which is crucial for protein assays. This combination makes the buffer ideal for various laboratory applications, particularly in immunological studies. -
Question:
Can I use 20x TBST Buffer for protein electrophoresis?
Answer: While 20x TBST Buffer is primarily designed for assays such as Western blotting, it’s not typically used for protein electrophoresis directly. Instead, it is often utilized in the post-transfer washing and dilution of antibodies during the Western blot process, after proteins have been separated via electrophoresis. Researchers find this buffer important for maintaining protein stability and ensuring effective antibody binding, which maximizes detection sensitivity and specificity in protein analysis. -
Question:
How does KCl in 20x TBST Buffer improve its performance?
Answer: The inclusion of KCl in 20x TBST Buffer enhances ionic strength, which is vital for protein stabilization and optimal binding during immunological assays. Higher ionic strength can improve the specificity of antibody binding, reduce background noise, and ensure a clearer signal detection. Researchers often notice improved results in immunodetection methods, making TBST ideal for applications such as Western blots, where maintaining protein activity and ensuring low non-specific binding is paramount. -
Question:
Is the formulation of 20x TBST Buffer compatible with ELISA assays?
Answer: Yes, 20x TBST Buffer is compatible with ELISA assays, where maintaining a consistent pH and ionic strength is crucial for assay performance. When diluted to 1x, it can be used for washing plates and diluting antibodies, helping to reduce non-specific binding. Researchers find that its use in ELISAs results in improved signal clarity and assay reliability, making it an excellent choice for various immunological assays seeking accurate quantification of proteins. -
Question:
Where can I buy 20x TBST Buffer (4 L, w/KCl) in Saudi Arabia?
Answer: You can buy 20x TBST Buffer (4 L, w/KCl) at Ubuy. Ubuy offers a variety of laboratory supplies, ensuring that you can conveniently find and order the buffer online. With its user-friendly interface and a wide selection of scientific materials, Ubuy is an efficient platform for purchasing laboratory reagents, making your shopping experience smoother and more efficient.
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Features & Benefits
- TRIS-buffered saline with Tween-20 (20x TBST) is ideal for Western blot and immunoassays.
- Acts as a pH stabilizer to maintain antibody-antigen binding during washes.
- Tween 20 minimizes nonspecific binding and enhances results.
- 10X TBST solution is convenient and ready to use after dilution.
- Clear, colorless liquid ensures high purity and efficacy.
- Formulated with ultrapure water for optimal performance.
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