What is Hanks Balanced Salt Solution (HBSS)?

Hanks Balanced Salt Solution, or HBSS, is a sterile saline solution commonly used in laboratories to provide essential nutrients to cells in culture. It helps maintain the pH and osmotic balance, mimicking the physiological conditions needed for various biological applications. Researchers often use HBSS in experiments involving cell viability, tissue disaggregation, or as a diluent for cells and reagents.

What is the purpose of using HBSS without calcium and magnesium?

Using HBSS without calcium and magnesium is particularly important in applications like cell dissociation or in experiments where reactivity with these ions could interfere with results. By eliminating these components, the solution minimizes the risk of unwanted cellular adhesion or activation, allowing for clearer assessments of cellular response in experiments involving growth factors or other reagents.

How is the 0.1m filtered sterilization of HBSS relevant?

The 0.1m filtered sterilization ensures that HBSS is free from microbial contamination, which is crucial for maintaining cell cultures and ensuring reliable experimental results. This level of filtration typically involves using a membrane filter that only allows liquid to pass while capturing bacteria and other organisms. This process ensures that research conclusions are based on accurate, uncontaminated data.

What are some practical applications of HBSS in a laboratory setting?

HBSS is widely used in various laboratory settings for cell culture maintenance, tissue processing, and sample preparation. It's ideal for washing cells prior to experimentation, serving as a diluent for cell suspensions, and for use in drug testing protocols where maintaining cellular integrity is essential. Researchers often utilize HBSS in applications ranging from pharmacology to toxicology testing.

Is HBSS ready to use upon purchase? What does this mean?

Yes, this specific HBSS product is ready to use straight out of the container, meaning it has been pre-prepared, sterilized, and is suitable for immediate application without the need for additional dilution or preparation steps. This convenience saves time and reduces the possibility of contamination during handling, allowing researchers to focus on their experiments.

Can I use HBSS for all cell types?

HBSS is versatile and can be used for various cell types, including adherent and suspension cells. However, it's essential to verify whether the specific cell line you are working with has unique requirements, such as specific nutrients or conditions. As such, intermediaries, especially in research settings, sometimes supplement HBSS with additional growth factors or serum to suit particular cell needs.

How should I store HBSS after opening?

After opening the HBSS, it is advisable to store it in a cool, dry place and tightly seal the container to prevent contamination. If the bottle is intact and handled properly, it can typically be used for some time without degradation. Always check for any signs of contamination or changes in clarity before each use, as this can indicate a compromised solution.

What is the volume of this HBSS solution, and is it available in different sizes?

This HBSS solution comes in a 1000ml bottle, which is suitable for numerous experiments and applications. While it is available in this size for significant usage, it is also offered in various other volumes by different manufacturers, allowing researchers to select a quantity based on their specific needs, whether for routine processes or large-scale experiments.

What safety precautions should I take when using HBSS?

While HBSS is generally safe to handle, it's important to wear appropriate personal protective equipment (PPE) such as gloves and lab coats to prevent any potential skin irritation. Additionally, using it in a well-ventilated area or fume hood can further minimize exposure risks, especially if mixed with other reagents. Properly disposing of any waste according to local regulations is also essential.

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CryoKing

Hanks' Balanced Salt Solution, HBSS Buffer Without Calcium & Magnesium, 0.1m Filtered Sterilization, Reay to Use Lab Buffer, 1000ml

Hanks' Buffer is the most commonly used balanced salt solution in biological experiments, user-friendly, and ensures the highest level of purity and reliability.

CryoKing

Item #: 224914999

Hanks' Balanced Salt Solution, HBSS Buffer Without Calcium & Magnesium, 0.1m Filtered Sterilization, Reay to Use Lab Buffer, 1000ml

Q: Where can I buy Hanks Balanced Salt Solution, HBSS Buffer Without Calcium & Magnesium, 0.1m Filtered Sterilization, Ready To Use Lab Buffer, 1000ml?

You can purchase Hanks Balanced Salt Solution, HBSS Buffer Without Calcium & Magnesium, from Ubuy in Saudi Arabia. Ubuy offers a straightforward online shopping experience and ensures access to high-quality laboratory products, making it easy to find the HBSS you need for your research and experiments.

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Item #: 224914999

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Hanks' Buffer is the most commonly used balanced salt solution in biological experiments, user-friendly, and ensures the highest level of purity and reliability.

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What Stands Out

Calcium-Free Formula

This HBSS Buffer is free from calcium and magnesium, making it ideal for sensitive cell culture applications where these ions may interfere with results.

Filtered Sterilization

Each 1000ml bottle is filtered to 0.1 microns, ensuring a sterile solution that eliminates contaminants, promoting reliable experimental outcomes.

Ready to Use

Pre-prepared and packaged for immediate use, this buffer saves time in laboratory settings, allowing researchers to focus on experimentation instead of preparation.

Product Details

Brand Name	CryoKing
Unit Count	1 Count
Manufacturer	BIOLOGIX
Included Components	Hanks Balanced Salt Solution
Part Number	HANKS-1000-1-New
Brand	CryoKing
Item Weight	2.2 lbs (1 kg)

What's in the box

Hanks Balanced Salt Solution

Who Should Buy?

Cell Culture
Ideal for researchers and labs conducting cell culture studies that require a stable environment without calcium and magnesium.
Tissue Preservation
Useful for laboratories needing a buffer to preserve tissues during handling and transportation for experiments.
Research Applications
Recommended for various biological experiments where a sterile, pH-balanced buffer solution is essential for accuracy.

Calcium-Magnesium Dependent Experiments
Not suitable for experiments that specifically require calcium and magnesium ions for proper biological reactions.

Product Description

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Question: How to Shop Hanks' Balanced Salt Solution, HBSS Buffer Without Online From Ubuy?

Answer: It’s easy to shop Hanks' Balanced Salt Solution, HBSS Buffer Without online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Hanks' Balanced Salt Solution, HBSS Buffer Without Available to Shop Online in Saudi Arabia?

Answer: Yes, at Ubuy Saudi Arabia this product is available for you to shop at a reasonable price. The Hanks' Balanced Salt Solution, HBSS Buffer Without is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

CryoKing Buffers Editorial Review

Hanks' Balanced Salt Solution, HBSS Buffer Without Calcium & Magnesium, is a high-quality lab buffer that is ideal for various biological applications. With a capacity of 1000ml per bottle and provided in a set of two, this buffer solution is convenient for multiple uses in a laboratory environment. Manufactured by BIOLOGIX under the CryoKing brand, it is designed to ensure optimal performance in cell culture and other scientific applications. The solution is filtered and sterilized, making it ready for immediate use without the need for further preparation. Users have appreciated the reliability and effectiveness of this buffer in experiments, making it a staple in many labs.

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Pros

Convenient 1000ml size per bottle
Filtered and sterilized for immediate use
Two bottles included per pack
Reliable for various biological applications
From a trusted manufacturer, BIOLOGIX

Cons

Weight may be slightly heavy for some users

Product Price History

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Features & Benefits

No calcium or magnesium ions to preserve trypsin activity.
Ideal for rinsing tissue blocks and cells in biological experiments.
Ready-to-use, no dilution or filtration required.
Minimized air exposure for high purity and reliability.
Long shelf life of 3 years for ongoing research needs.
Perfect for routine applications in cell culture.

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Hanks' Balanced Salt Solution, HBSS Buffer Without Calcium & Magnesium, 0.1m Filtered Sterilization, Reay to Use Lab Buffer, 1000ml

SAR 191

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