10x TBS Buffer (1 L, w/KCl)
TRIS-buffered saline (10x TBS) acts as a pH stabilizer, allowing for washing without disruption of antibody-antigen binding interactions.
10x TBS Buffer (1 L, w/KCl)
منتج #: 125665253

10x TBS Buffer (1 L, w/KCl)

منتج #: 125665253

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    TRIS-buffered saline (10x TBS) acts as a pH stabilizer, allowing for washing without disruption of antibody-antigen binding interactions.
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    مايفيد

    Concentration
    Highly concentrated 10x TBS buffer allows for easy dilution, ensuring versatility in various laboratory applications to meet specific experimental needs.
    Potassium Chloride
    Contains KCl to maintain ionic strength, stabilizing protein interactions and enhancing performance in biochemical assays compared to standard buffers.
    Volume Convenience
    Offered in a 1-liter volume, ideal for high-throughput applications, reducing the frequency of ordering and preparation for busy laboratories.

    تفاصيل المنتج

    Shop 10x TBS Buffer (1 L, w/KCl) online at a best price in السعودية. B0DJCQ23R7
    Brand NameGeneric
    ManufacturerBioland Scientific LLC
    Included Componentsbuffer
    Model NumberTBS03-1L
    Part NumberTBS03-1L
    Item Weight2.2 lbs (1 kg)

    من يجب أن يشتري؟

    Suitable For
    • Molecular Biologists

      Ideal for PCR, western blotting, and other applications requiring stable pH and ionic strength.

    • Research Laboratories

      Essential for experiments needing consistent buffer conditions to ensure reproducibility of results.

    • Academic Institutions

      Useful for teaching purposes in biochemistry and molecular biology courses, enhancing practical lab experiences.

    Not Suitable For
    • Beginner Users

      May be challenging for novices who lack experience with buffer preparations and biochemical applications.

    وصف المنتج

    10x TBS Buffer (1 L, w/KCl)

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    أسئلة العملاء & الإجابات

    • سؤال: What is 10x TBS Buffer (1 L, w/KCl) used for?

      إجابه: 10x TBS Buffer is widely utilized in molecular biology laboratories primarily for the preparation of dilutions and buffer systems in various experiments. Its formulation includes Tris and NaCl, which help maintain a stable pH and ionic strength, providing an optimal environment for biochemical reactions. It is commonly employed in western blots, DNA/RNA extraction, and other applications where maintaining the integrity of nucleic acids or proteins is critical.
    • سؤال: How do I prepare 1x TBS Buffer from 10x TBS Buffer?

      إجابه: To prepare a 1x TBS Buffer from a 10x solution, carefully dilute the buffer by mixing one part of 10x TBS with nine parts of distilled water. For instance, if you need 100 mL of 1x TBS, combine 10 mL of 10x TBS with 90 mL of distilled water. This ensures you achieve the desired concentration for your experiments, maintaining optimal conditions for reactions.
    • سؤال: What is the role of KCl in 10x TBS Buffer?

      إجابه: KCl in 10x TBS Buffer contributes to the buffer's ionic strength, which is crucial in stabilizing macromolecules like proteins and nucleic acids during various biological assays. This enhances the buffer's ability to maintain a consistent pH, ensuring that enzymatic reactions occur effectively. The presence of potassium ions also helps with the stabilization of nucleic acids, making it a valuable component in molecular biology applications.
    • سؤال: Can 10x TBS Buffer be used for cell culture applications?

      إجابه: While 10x TBS Buffer is not primarily designed for cell culture, it can be utilized for washing and resuspending cells during experiments involving protein analysis. Proper dilution is essential, as high concentrations may negatively impact cell viability. In a typical application, researchers may use diluted TBS buffer to wash cells before lysis in protein extraction protocols.
    • سؤال: Is 10x TBS Buffer compatible with enzyme assays?

      إجابه: Yes, 10x TBS Buffer is compatible with a variety of enzyme assays. Its buffered nature provides a stable pH necessary for enzyme activity in biochemical reactions. However, to avoid interference, it is recommended to assess compatibility with specific enzymes, as certain reactions may be sensitive to buffer components. Researchers often use this buffer in enzyme-linked immunosorbent assays (ELISAs) and other similar applications.
    • سؤال: What precautions should I take when handling 10x TBS Buffer?

      إجابه: When handling 10x TBS Buffer, it is essential to wear appropriate personal protective equipment (PPE), including gloves and goggles, to avoid skin and eye contact. Additionally, ensure that the buffer is stored properly, away from direct sunlight and at room temperature, to maintain its integrity. Following standard laboratory safety protocols will help prevent contamination and ensure accurate experimental results.
    • سؤال: How long can I store 10x TBS Buffer?

      إجابه: 10x TBS Buffer can typically be stored for several months when kept in appropriate conditions. Store it at room temperature and protect it from light to prevent degradation. Always ensure that the container is tightly sealed to avoid contamination. If there are visible changes in color or turbidity, it is advisable to prepare a fresh solution for optimal experimental performance.
    • سؤال: Does 10x TBS Buffer affect protein stability?

      إجابه: 10x TBS Buffer is formulated to maintain protein stability by providing a controlled pH and ionic environment. The buffer's components help minimize degradation and denaturation of proteins during experiments. Researchers frequently use this buffer in procedures such as western blotting and protein assays where preserving protein integrity is crucial for obtaining reliable results.
    • سؤال: Where can I buy 10x TBS Buffer (1 L, w/KCl) in Saudi Arabia?

      إجابه: You can buy 10x TBS Buffer (1 L, w/KCl) from Ubuy. They offer a reliable platform for laboratory supplies, ensuring that you have access to quality products readily available in Saudi Arabia. Simply visit their website and search for the buffer to make your purchase.

    Buffers TBS03-1L Editorial Review

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